This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
Llewellyn, S. V., Conway, G. E., Shah, U., Evans, S. J., Jenkins, G. J., Clift, M. J., Doak, S. H. Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. J. Vis. Exp. (160), e61141, doi:10.3791/61141 (2020).