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Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking
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JoVE Journal Biochimica
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking

7,943 Views

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07:49 min

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June 08, 2020

DOI:

10.3791/60950-v

DOI:
10.3791/60950-v

•

07:49 min
June 08, 2020

•

5 Views
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1School of Physics and Astronomy,University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College

Summary

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Conventionele BODIPY conjugaten kunnen worden gebruikt voor live-cel single-molecule lokalisatie microscopie (SMLM) door de exploitatie van hun tijdelijk vormende, rood verschoven grond staat dimers. We presenteren een geoptimaliseerd SMLM-protocol om subcellulaire neutrale lipiden en vetzuren in levende zoogdier- en gistcellen op nanoscopische lengteschaal te volgen en op te lossen.

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Cite this Article

Adhikari, S., Banerjee, C., Moscatelli, J., Puchner, E. M. Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking. J. Vis. Exp. (160), e60950, doi:10.3791/60950 (2020).

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