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用于活细胞超分辨率显微镜和单分子跟踪的传统 BODIPY 结合物
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Biochimica
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JoVE Journal Biochimica
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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用于活细胞超分辨率显微镜和单分子跟踪的传统 BODIPY 结合物

7,943 Views

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07:49 min

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June 08, 2020

DOI:

10.3791/60950-v

DOI:
10.3791/60950-v

•

07:49 min
June 08, 2020

•

5 Views
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1School of Physics and Astronomy,University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College

Summary

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传统的BODIPY结合物可用于活细胞单分子定位显微镜（SMLM），其瞬时成型、红移地面状态变暗器。我们提出了优化的SMLM协议，以跟踪和解决活哺乳动物和酵母细胞中的亚细胞中性脂质和脂肪酸在纳米长度尺度上。

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Cite this Article

Adhikari, S., Banerjee, C., Moscatelli, J., Puchner, E. M. Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking. J. Vis. Exp. (160), e60950, doi:10.3791/60950 (2020).

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