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Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

DOI:
10.3791/60926-v

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08:28 min

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March 10, 2020

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, ,
1Department of Biomedical Engineering,Michigan State University, 2Institute for Quantitative Health Sciences and Engineering,Michigan State University

Capitoli

  • 00:05Introduction
  • 00:56Embryonic Heart Harvest
  • 03:09Tissue Preparation
  • 04:05Cryostat Sectioning
  • 05:31Qualitative Heart Defect and Cardiac Muscle Compaction Analysis
  • 07:02Results: Representative Muscle Compaction Analyses
  • 07:32Conclusion

Summary

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In this protocol, we describe procedures to qualitatively and quantitatively analyze developmental phenotypes in mice associated with congenital heart defects.

Tags

Congenital Heart DefectsMouse EmbryosHistological AnalysisQualitative AnalysisQuantitative AnalysisDevelopmental CardiologyCardiology ResearchEmbryonic Heart CollectionHeart Fixation

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