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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
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March 12, 2020
DOI:
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, 2Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz
Summary
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The protocol presents a complete validated TNBS-induced murine model of Crohn's disease and methods for visualization of estrogen receptors by immunohistochemistry using immunofluorescence of formalin-fixed colon sections embedded in paraffin.
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Jacenik, D., Zielińska, M., Michlewska, S., Fichna, J., Krajewska, W. M. Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence. J. Vis. Exp. (157), e60813, doi:10.3791/60813 (2020).
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