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Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
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Medicina
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JoVE Journal Medicina
Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

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06:29 min

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May 02, 2020

DOI:

10.3791/60812-v

DOI:
10.3791/60812-v

•

06:29 min
May 02, 2020

•

3 Views
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1Alcon Vision, LLC, 2Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science,University of Waterloo

Summary

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The purpose of this protocol is to evaluate if different artificial tear formulations can protect human corneal epithelial cells from desiccation using an in vitro model. After exposure to artificial tear formulations and desiccation, human corneal epithelial cells are assessed for metabolic activity.

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Cite this Article

Rangarajan, R., Ketelson, H. A., Do, R., McCanna, D. J., Suko, A., Enstone, D., Subbaraman, L. N., Dantam, J., Jones, L. W. Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation. J. Vis. Exp. (159), e60812, doi:10.3791/60812 (2020).

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