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08:34 min
February 05, 2020
DOI:
10.3791/60774-v
在这里,我们提出了一个方案,通过酶形成蛋白质聚合物与受控序列结合蛋白质单体,并将其固定在表面进行单分子力光谱研究。
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Deng, Y., Zheng, B., Liu, Y., Shi, S., Nie, J., Wu, T., Zheng, P. OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy. J. Vis. Exp. (156), e60774, doi:10.3791/60774 (2020).
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