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用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析
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Genetica
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JoVE Journal Genetica
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
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用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析

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12:18 min

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February 01, 2020

DOI:

10.3791/60705-v

DOI:
10.3791/60705-v

•

12:18 min
February 01, 2020

•

1 Views
, , ,
1Department of Oral and Maxillofacial Diseases,University of Helsinki, 2Helsinki University Hospital

Summary

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这里描述的是使用甲基化特异性探针扩增方法分析使用骨肉瘤衍生的细胞外囊泡治疗的中位体干细胞中LINE-1元素的甲基化水平。也演示了超中心化，一种从胎儿牛血清中分离细胞外囊泡的常用程序。

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LINE-1 MethylationMesenchymal Stem CellsOsteosarcomaExtracellular VesiclesDNA MethylationEpigeneticsUltracentrifugationEV IsolationFBS Depletion

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Cite this Article

Sinha, S., Mannerström, B., Seppänen-Kaijansinkko, R., Kaur, S. LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles. J. Vis. Exp. (156), e60705, doi:10.3791/60705 (2020).

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