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JoVE Journal Genetics
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
 

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Article DOI: 10.3791/60705-v • 12:18 min • February 1st, 2020
February 1st, 2020 •
*1,2, *1,2, 1,2, 1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital
* These authors contributed equally

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Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.

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LINE-1 Methylation Analysis Mesenchymal Stem Cells Osteosarcoma-derived Extracellular Vesicles DNA Methylation Epigenetic Signatures Aberrant Gene Expression Genomic Abnormalities Long Interspersed Nuclear Elements Hypermethylation Cancer Chromosomal Instability Cancer EVs MECs Fetal Bovine Serum Mammalian Cell Culture Media Bovine-derived EVs EV-depleted FBS Ultracentrifuge Tubes Swinging Rotor Supernatant Dark Brown Pellet
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