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Journal / Immunology and Infection / Analyzing the Permeability of the Blood-Brain…
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Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
 

Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells

Article DOI: 10.3791/60692-v • 06:26 min • February 14th, 2020
February 14th, 2020 •
1, 1, 1, 1
1Institute of Physiological Chemistry, Martin Luther University Halle-Wittenberg

Capitoli

Riepilogo
Automatic Translation

The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.

Tags

Permeability Blood-Brain Barrier Microbial Traversal Microvascular Endothelial Cells Bacterial Meningitis Postoperative Delirium Treatment Effects High Throughput Analysis 12-well Plate Collagen IV Fibronectin Mixture Cell Culture Incubator DMEM F12 Medium Human Microvascular Endothelial Cells
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