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通过2',7'-二氯二氢氟辛二乙酸染色检测粘附细胞中的总活性氧物种
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Detection of Total Reactive Oxygen Species in Adherent Cells by 2′,7′-Dichlorodihydrofluorescein Diacetate Staining

通过2',7'-二氯二氢氟辛二乙酸染色检测粘附细胞中的总活性氧物种

DOI:

04:53 min

June 23, 2020

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Capitoli

  • 00:04Introduction
  • 00:32Cell Seeding and DCFH-DA Solution Preparation
  • 01:32DCFH-DA Staining
  • 02:12Image Acquisition and Intensity Measurement
  • 03:29Results: Iron Treatment Increases ROS in Colorectal Cancer Cells
  • 04:07Conclusion

Summary

Traduzione automatica

在这里,我们提出了一个协议,使用2',7'-二氯二氢氟辛二乙酸酯(DCFH-DA)检测细胞活性氧物种(ROS)。2',7'-二氯二氢氟酸酯(DCFH-DA)。该方法使用荧光显微镜可视化粘附细胞中的细胞ROS定位,并用荧光板读取器量化ROS强度。该协议简单、高效且经济高效。

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