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Immunology and Infection

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JoVE Journal Immunology and Infection
Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy
 

Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy

Article DOI: 10.3791/60509-v • 08:11 min • April 15th, 2020
April 15th, 2020 •
*1, *1, 1, 1, 1, 1, 1
1Department of Neonatology, Heidelberg University Children's Hospital
* These authors contributed equally

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Here, we show how to perform intravital microscopy on post-capillary venules of the mouse cremaster muscle. Commonly applied to different models of inflammation and sepsis, particularly those induced by chemokines and cytokines, we highlight its relevance in the study of muscolopathies involving exaggerated muscular leukocyte infiltration.

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Leukocyte Infiltration Cremaster Muscle Mice Intravital Microscopy Endothelium Leukocyte Interactions Leukocyte Recruitment Physiological Processes Pathophysiological Processes Inflammatory Models Septic Models Musculopathies Hands-on Experience Minor Deviations Experimental Animal Dorsal Recumbent Position Heating Pad Suture Incision Neck Upper Thorax Muscle Dissection
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