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Whole-Mount In Situ Hybridization i Zebrafish Embryoner og Tube Formation Assay i iPSC-ECs at studere den rolle, Endoglin i vaskulær udvikling
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JoVE Journal Biologia dello sviluppo
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Whole-Mount In Situ Hybridization i Zebrafish Embryoner og Tube Formation Assay i iPSC-ECs at studere den rolle, Endoglin i vaskulær udvikling

DOI:
10.3791/60498-v

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08:27 min

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May 28, 2020

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, , , , , ,
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University

Capitoli

  • 00:04Introduction
  • 01:00Zebrafish Embryo Collection and Fixation
  • 02:55Amplification and Microscopy
  • 04:27Tube Formation Assay
  • 06:40Results: Defective Tube Formation After Endoglin Knockdown
  • 07:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

Præsenteret her er en protokol for hele mount in situ RNA hybridisering analyse i zebrafisk og rør dannelse assay i patient-afledt induceret pluripotente stamceller-afledte endotelceller til at studere den rolle, endoglin i vaskulær dannelse.

Tags

Whole-mount In Situ HybridizationWISHZebrafish EmbryosTube Formation AssayIPSC-ECsEndoglinVascular DevelopmentMicroinjectionMorpholinosMRNAProteinase KTarget ProbesParaformaldehydeSSCTAmp 1Amp 2

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