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Um ensaio de carga bacteriana molecular de tuberculose (TB-MBLA)
This content is Free Access.
1Division of Infection and Global Health, School of Medicine,University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences,University of Glasgow
Capitoli
- 00:05Introduction
- 01:33Sample Preparation and Heat Inactivation
- 02:30RNA Extraction
- 06:28Reverse Transcriptase qPCR
- 07:57Results: Heat Inactivation of M. tuberculosis Bacilli
- 09:43Conclusion
Descrevemos um teste de ensaio de carga bacteriana molecular de tuberculose realizado após a inativação do calor da escória. A inativação do calor torna as amostras de espelo não infecciosas e evita a necessidade de laboratórios de nível de contenção 3 para testes moleculares de tuberculose.
Tags
Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)TuberculosisDisease BurdenAnti-tuberculosis MedicationTreatment ResponseQuantitative ResultNontuberculous MycobacteriaChronic Obstructive Pulmonary DiseaseBacille Calmette-Guerin (BCG)Sputum SamplesRNA ExtractionPhenol ChloroformExtraction Control
