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Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
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Biochimica
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JoVE Journal Biochimica
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

DOI:
10.3791/60155-v

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06:17 min

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August 15, 2019

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, , , , , ,
1Department of Microbiology,Universität Innsbruck

Capitoli

  • 00:04Titolo
  • 00:39Preparation of Medium and Filling of Cultivation Flasks
  • 02:29Inoculation of the Medium
  • 03:38Incubation, Sampling, and Analysis
  • 04:57Results: Microorganism Cultivation under Anaerobic Conditions
  • 05:48Conclusion

Summary

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As obligate anaerobic organisms are unable to grow upon oxygen exposure, the use of anaerobic culturing techniques is indispensable. Here, we demonstrate a simple and effective method to cultivate a mixed culture derived from a biogas plant from media preparation to gas and volatile fatty acid quantification.

Tags

Keywords: AnaerobicAnoxicOxygen-freeREDOX IndicatorReducing AgentAnaerobic DigesterSludgeSerum FlaskInoculumLiquid CultureMedium PreparationMicroorganism Cultivation

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