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Neuroscience

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JoVE Journal Neuroscience
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
 

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Article DOI: 10.3791/60118-v • 10:09 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1
1U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Univ. Lille, INSERM

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The present report highlights chronological requirements for extracellular vesicle (EV) isolation from microglia or blood macrophages. Microglia-derived EVs were evaluated as regulators of the neurite outgrowth while blood macrophage-derived EVs were studied in the control of C6 glioma cell invasion in in vitro assays. The goal is to better understand these EV functions as immune mediators in specific microenvironments.

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Immune Cell-derived Extracellular Vesicles Functional Impact Cell Environment Isolation Protocol EBDF Proteins Integrity And Vertical Activities LC (liquid Chromatography) Pathological Conditions Biological Ethics Culture Experiments Size Exclusion Chromatography Column Nanoparticle Counter Mass Spectrometer Antonella Raffo-Romero Pre-isolation Of EVs Microglia Cultures Macrophage Cultures Centrifugation
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