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Reprogramação hemogénica de fibroblastos humanos pela expressão forçada de fatores de transcrição
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Reprogramação hemogénica de fibroblastos humanos pela expressão forçada de fatores de transcrição

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11:42 min

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November 04, 2019

DOI:

10.3791/60112-v

DOI:
10.3791/60112-v

•

11:42 min
November 04, 2019

•

4 Views
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1Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, 2Wallenberg Center for Molecular,Lund University, 3Center for Neuroscience and Cell Biology,University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Summary

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Este protocolo demonstra a indução de um programa hemogenic em fibroblastos dérmicos humanos pela expressão forçada dos fatores de transcrição GATA2, GFI1B e FOS para gerar células-tronco e progenitoras hematopoiéticas.

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Cite this Article

Silvério-Alves, R., Gomes, A. M., Kurochkin, I., Moore, K. A., Pereira, C. Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors. J. Vis. Exp. (153), e60112, doi:10.3791/60112 (2019).

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