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Hemogene herprogrammering van menselijke fibroblasten door afgedwongen expressie van transcriptiefactoren
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
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Hemogene herprogrammering van menselijke fibroblasten door afgedwongen expressie van transcriptiefactoren

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11:42 min

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November 04, 2019

DOI:

10.3791/60112-v

DOI:
10.3791/60112-v

•

11:42 min
November 04, 2019

•

4 Views
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1Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, 2Wallenberg Center for Molecular,Lund University, 3Center for Neuroscience and Cell Biology,University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Summary

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Dit protocol demonstreert de inductie van een hemogeen programma in menselijke dermale fibroblasten door afgedwongen expressie van de transcriptiefactoren GATA2, GFI1B en FOS om hematopoietische stam en voorlopercellen te genereren.

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Cite this Article

Silvério-Alves, R., Gomes, A. M., Kurochkin, I., Moore, K. A., Pereira, C. Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors. J. Vis. Exp. (153), e60112, doi:10.3791/60112 (2019).

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