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Biochemistry

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JoVE Journal Biochemistry
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
 

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

Article DOI: 10.3791/59994-v • 08:21 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 1
1Graduate School of Medicine, Korea University

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GPCR-β-arrestin interactions are an emerging field in GPCR drug discovery. Accurate, precise and easy to set up methods are necessary to monitor such interactions in living systems. We show a structural complementation assay to monitor GPCR-β-arrestin interactions in real time living cells, and it can be extended to any GPCR.

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Keywords: GPCR β-arrestin1/2 Real-time Live-cell Drug Discovery Receptor Pharmacology PCR Cloning Restriction Digestion High-fidelity DNA Polymerase Opioid Receptors Dopamine Receptors Neurology Cardiopulmonary Disease
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