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09:48 min
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September 26, 2019
DOI:
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation,University of Miami Miller School of Medicine, 2Batchelor Children’s Research Institute,University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine, 5Holtz Children’s Hospital,University of Miami Miller School of Medicine
Summary
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该协议的目标是通过使用胶原酶对组织进行酶消化，分离位于结肠层状的单核细胞。该协议允许对单核细胞进行有效分离，从而产生单个细胞悬浮液，进而可用于强健的免疫分体。
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Cite this Article
McManus, D., Novaira, H. J., Hamers, A. A., Pillai, A. B. Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E. J. Vis. Exp. (151), e59821, doi:10.3791/59821 (2019).
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