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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析

JoVE Journal
Biologia dello sviluppo

This content is Free Access.

JoVE Journal Biologia dello sviluppo

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析

8,009 Views

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09:12 min

•

September 16, 2019

DOI:

10.3791/59700-v

DOI:

10.3791/59700-v

•

09:12 min

September 16, 2019

•

31 Views

Kinga Winczura¹, Pawel Grzechnik¹

¹School of Biosciences,University of Birmingham

Summary

在这里，我们提出了RNADNA依赖性裂解的协议。这使得RNA2'-O-甲基化能够进行快速和位点依赖性分析。这种方法可用于对打用RNA活性的初步或主要评估。

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