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标准化和可扩展的测定，以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽
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November 06, 2019
DOI:
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine,Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics,Leiden University, 3Ncardia, 4Department of Anatomy and Embryology,Leiden University Medical Center, 5Mimetas
Summary
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该方法将iPSC衍生内皮细胞的培养描述为标准化微流体平台中的40个注入3D微容器。该平台支持在3D中研究梯度驱动的血管生成，包括以可扩展的高通量方式血管生成芽的血管生成和稳定。
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Cite this Article
van Duinen, V., Stam, W., Borgdorff, V., Reijerkerk, A., Orlova, V., Vulto, P., Hankemeier, T., van Zonneveld, A. J. Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro. J. Vis. Exp. (153), e59678, doi:10.3791/59678 (2019).
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