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フローサイトメトリーによる生体内におけるタンパク質自己集合体の検出と特性特性
JoVE Journal
Biologia
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JoVE Journal Biologia
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
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フローサイトメトリーによる生体内におけるタンパク質自己集合体の検出と特性特性

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05:58 min

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July 17, 2019

DOI:

10.3791/59577-v

DOI:
10.3791/59577-v

•

05:58 min
July 17, 2019

•

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, , , ,
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Summary

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本稿では、S.セレビシエ細胞とHEK293T細胞の両方でタンパク質自己集合を定量するFRETベースのフローサイトメトリープロトコルについて説明する。

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Cite this Article

Venkatesan, S., Kandola, T. S., Rodríguez-Gama, A., Box, A., Halfmann, R. Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry. J. Vis. Exp. (149), e59577, doi:10.3791/59577 (2019).

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