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Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue
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JoVE Journal Chimica
Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

DOI:
10.3791/59471-v

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07:09 min

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May 09, 2019

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, , , , ,
1Department of Biological and Environmental Sciences,Longwood University, 2Department of Chemistry and Physics,Longwood University

Capitoli

  • 00:04Titolo
  • 00:47Restriction Enzyme Digestion
  • 01:50Ligation
  • 02:29Colony PCR
  • 03:14Titrating E. coli to Check Function of Device
  • 03:54Using E. coli as Chemical Sensor for GSR
  • 04:44Fluorescence Analysis Using Portable Spectrometer
  • 05:09Fluorescence Analysis Using 96-well Plate Reader
  • 05:34Results: Analysis of the Biosensor and Presumptive Detection of Gunshot Residue
  • 06:29Conclusion

Summary

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A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

Tags

Bacterial BiosensorGunshot ResidueForensic ApplicationsSynthetic BiologyPlasmid DNARestriction EnzymesDNA LigationBacterial TransformationPCR

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