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DREADD控制神经活动慢性操作的非侵入性策略
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Neuroscienze
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JoVE Journal Neuroscienze
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity
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DREADD控制神经活动慢性操作的非侵入性策略

12,714 Views

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00:08 min

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August 25, 2019

DOI:

10.3791/59439-v

DOI:
10.3791/59439-v

•

00:08 min
August 25, 2019

•

23 Views
, , , ,
1Section on Light and Circadian Rhythms (SLCR),National Institute of Mental Health, 2Howard Hughes Medical Institute, Department of Neuroscience,Scripps Research Institute

Summary

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在这里，我们描述了两种非侵入性方法，使用小鼠的化学遗传学来长期控制神经元活动。眼药水用于每天提供氯扎平-N-氧化物（CNO）。我们还描述了在饮用水中长期使用CNO的两种方法。这些慢性神经元控制策略需要最少的干预，以减少动物的压力。

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Cite this Article

Zhan, J., Komal, R., Keenan, W. T., Hattar, S., Fernandez, D. C. Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity. J. Vis. Exp. (150), e59439, doi:10.3791/59439 (2019).

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