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3D-billeddannelse i høj opløsning af rabies virus infektion i et solvent renset hjernevæv
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JoVE Journal Neuroscienze
High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

3D-billeddannelse i høj opløsning af rabies virus infektion i et solvent renset hjernevæv

DOI:
10.3791/59402-v

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09:42 min

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April 30, 2019

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, , , ,
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut,Federal Research Institute for Animal Health

Capitoli

  • 00:04Titolo
  • 00:36Brain Tissue Sample Fixation and Sectioning
  • 01:26Sample Pretreatment with Methanol
  • 02:23Immunostaining
  • 04:03Nuclear Staining and Tissue Clearing
  • 05:08Sample Mounting
  • 06:08Imaging and Image Processing
  • 07:50Results: Representative Deep Tissue Staining of Rabies Virus-Infected Brain Tissue Samples
  • 09:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

Roman, immun farvning-kompatible vævs clearing teknikker som den ultimative 3D-billeddannelse af solvent-clearede organer tillader 3D visualisering af rabiesvirus hjerneinfektion og dens komplekse cellulære miljø. Tykke, antistof-mærkede hjernevæv skiver er lavet optisk gennemsigtige for at øge billeddannelse dybde og for at muliggøre 3D-analyse af konfokale Laserscanning mikroskopi.

Tags

Keywords: 3D ImagingRabies VirusImmunostainingSolvent ClearingVirus InfectionCellular ContextVibratome SectioningMethanol PretreatmentBleachingImmunostainingPermeabilizationBlockingPrimary Antibody Labeling

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