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High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
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Capitoli
- 00:04Titolo
- 00:36Brain Tissue Sample Fixation and Sectioning
- 01:26Sample Pretreatment with Methanol
- 02:23Immunostaining
- 04:03Nuclear Staining and Tissue Clearing
- 05:08Sample Mounting
- 06:08Imaging and Image Processing
- 07:50Results: Representative Deep Tissue Staining of Rabies Virus-Infected Brain Tissue Samples
- 09:08Conclusion
Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.
