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Journal / Genetics / Isolation of Papillary and Reticular Fibroblasts…
JoVE Journal
Genetics

This Contenuto is Open Access.

JoVE Journal Genetics
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
 

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Article DOI: 10.3791/59372-v • 09:14 min • May 7th, 2019
May 7th, 2019 •
*1, *1, 1
1Skin, Endothelium Research Division, Department of Dermatology, Medical University of Vienna
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This manuscript describes a FACS-based protocol for isolation of papillary and reticular fibroblasts from human skin. It circumvents in vitro culture which was inevitable with the commonly used isolation protocol via explant cultures. The emanating fibroblast subsets are functionally distinct and display differential gene expression and localization within the dermis.

Tags

Isolation Papillary Fibroblasts Reticular Fibroblasts Human Skin Fluorescence-activated Cell Sorting Cell Surface Marker Expression Functionally Distinct In Vitro Manipulation Isolation Methods Explant Cultures Dermal Fibroblast Subsets Homeostatic Conditions Skin Pathologies Cancer Inflammatory Skin Diseases Human Tissue Handling Cell Yields Visual Demonstration Full-thickness Dermis Subcutaneous Fat Layer Scalpel Tissue Slicing
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