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Isolasjon, overføring og Prion Protein uttrykk under Neuronal differensiering av menneskelig tannlegekontoret masse stammen celler
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Capitoli

  • 00:04Titolo
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Traduzione automatica

Her presenterer vi en protokoll for menneskelig Dental stamceller fra cellulose isolasjon og overføring for å vurdere prion protein uttrykk under neuronal differensiering prosessen.

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