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Isolement, Propagation et Expression de la protéine Prion au cours de la différenciation neuronale de cellules souches humaines pulpe dentaire
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Capitoli

  • 00:04Titolo
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Traduzione automatica

Nous présentons ici un protocole pour humain isolement des cellules souches pulpe dentaire et propagation afin d’évaluer l’expression de la protéine prion au cours du processus de différenciation neuronale.

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