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In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression
JoVE Journal
Genetica
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JoVE Journal Genetica
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression

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08:54 min

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March 29, 2019

DOI:

10.3791/59235-v

DOI:
10.3791/59235-v

•

08:54 min
March 29, 2019

•

1 Views
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1Center for Epigenetics,Van Andel Research Institute, 2Amgen

Summary

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Denne protokol beskriver de nødvendige skridt for at generere en modelsystem hvor transkription af en endogen gen af interesse betinget kan styres i levende dyr eller celler ved hjælp af forbedrede lac repressor og/eller tet aktivator systemer.

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Cite this Article

Vander Schaaf, N. A., Oghamian, S., Park, J., Kang, L., Laird, P. W., Lee, K. In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression. J. Vis. Exp. (145), e59235, doi:10.3791/59235 (2019).

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