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培养细胞功能化纳米化对跨 golgi 网络的内分泌吸收和逆行迁移分析
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February 21, 2019
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蛋白质从细胞表面逆行运输到 golgi 是维持膜稳态所必需的。在这里, 我们描述了一种方法, 生物化学分析细胞地对golgi 的重组蛋白运输使用功能化纳米棒在海拉细胞。
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Buser, D. P., Spiess, M. Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells. J. Vis. Exp. (144), e59111, doi:10.3791/59111 (2019).
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