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Biochemistry

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JoVE Journal Biochemistry
Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
 

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Article DOI: 10.3791/59111-v • 11:05 min • February 21st, 2019
February 21st, 2019 •
1, 1
1Biozentrum, University of Basel

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Retrograde transport of proteins from the cell surface to the Golgi is essential to maintain membrane homeostasis. Here, we describe a method to biochemically analyze cell surface-to-Golgi transport of recombinant proteins using functionalized nanobodies in HeLa cells.

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Endocytic Uptake Retrograde Transport Trans-Golgi Network Functionalized Nanobodies Cultured Cells Protein Transport Sulfation Post-translation Modification DTN Compartment Life Cell Imaging Electron Microscopy Endocytic Processes Disease-related Cell Surface Receptors Multicellular Organisms Bacterial Colony LB Medium Antibiotics Protein Traffic
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