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Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

DOI:
10.3791/58978-v

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08:04 min

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February 08, 2019

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, , , , ,
1Department of Marine Biotechnology and Resources,National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Capitoli

  • 00:04Titolo
  • 00:47Neisseria gonorrhoeae (GC) Strain Maintenance
  • 01:31GC Aggregate Quantification
  • 03:13GC Live/Dead Aggregate Analysis
  • 04:26Image Analysis
  • 05:48Results: Representative GC Aggregation Survival Before and After Antibiotic Treatment
  • 07:21Conclusion

Summary

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A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.

Tags

Neisseria GonorrhoeaeAntibiotic SusceptibilityATP-utilization AssayLive/dead StainingBacterial AggregatesCeftriaxoneMicrobial CultureOptical DensitySonication

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