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Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

8,465 Views

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08:04 min

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February 08, 2019

DOI:

10.3791/58978-v

DOI:
10.3791/58978-v

•

08:04 min
February 08, 2019

•

9 Views
, , , , ,
1Department of Marine Biotechnology and Resources,National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Summary

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A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.

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Neisseria GonorrhoeaeAntibiotic SusceptibilityATP-utilization AssayLive/dead StainingBacterial AggregatesCeftriaxoneMicrobial CultureOptical DensitySonication

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Wang, L., Wagner, J., Capino, A., Nesbit, E., Song, W., Stein, D. C. Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining. J. Vis. Exp. (144), e58978, doi:10.3791/58978 (2019).

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