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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:
10.3791/58952-v

•

08:59 min
February 12, 2019

•

11 Views
, , , , ,
1Cellular Protein Chemistry,Utrecht University, 2Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Summary

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在这里, 我们描述了一种一般脉冲追逐方法的协议, 该方法允许在活细胞中对蛋白质的折叠、迁移和降解进行动力学分析。

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Cite this Article

McCaul, N., Yeoh, H. Y., van Zadelhoff, G., Lodder, N., Kleizen, B., Braakman, I. Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase. J. Vis. Exp. (144), e58952, doi:10.3791/58952 (2019).

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