Rivista
/
Immunologia e infezioni
/
增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多
JoVE Journal
Immunologia e infezioni
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis
Please note that all translations are automatically generated. Click here for the English version.

增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多

DOI:
10.3791/58751-v

•

12:35 min

•

January 04, 2019

•

, , , ,
1Anesthesiology Department,The Second Hospital of Dalian Medical University, 2Scientific Research Center,The Second Hospital of Dalian Medical University

Capitoli

  • 00:00Titolo
  • 01:40Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression
  • 04:28Mouse Peritoneal Macrophage Isolation and Primary Culture
  • 06:57Macrophage Phagocytosis Assay using Flourescence Microscopy
  • 08:28Macrophage Phagocytosis Assay using Flow Cytometer
  • 11:05Risultati
  • 11:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个评估小鼠腹腔巨噬细胞吞噬使用增强绿色荧光蛋白表达大肠杆菌的方案.

Tags

EGFP-expressing Escherichia ColiPhagocytosisMacrophagesPeritoneal MacrophagesInnate Immune FunctionAgingFluorescence MicroscopyFlow CytometryTA CloningPET SUMO VectorBL21 Competent CellsKanamycin

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

小鼠腹膜腔细胞的分离

Neuropathogenic的非侵入型<em>大肠杆菌</em>感染乳鼠

高通量荧光技术巨噬细胞吞噬功能和肌动蛋白聚合的评估

巨噬细胞胆固醇消耗及其对的吞噬作用<em>新型隐球菌</em

一步法负色谱纯化<em>幽门螺杆菌</em>中性粒细胞激活蛋白过度表达<em>大肠杆菌</em>在批处理模式

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

人类的过表达和纯化<em>顺</emβ-肾上腺素转移酶<em>大肠杆菌</em

利用共焦显微镜可视化巨噬细胞胞外陷阱

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

在大肠杆菌中生成特异的乙酰蛋白的简易协议

Read Article