Rivista
/
Genetica
/
Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
JoVE Journal
Genetica
This content is Free Access.
JoVE Journal Genetica
Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

8,898 Views

•

13:07 min

•

January 30, 2019

DOI:

10.3791/58715-v

DOI:
10.3791/58715-v

•

13:07 min
January 30, 2019

•

3 Views
, ,
1Department of Infectious Diseases, School of Immunology & Microbial Sciences,King’s College London

Tags

HIV-1 Reverse TranscriptionNascent Single-stranded Viral DNA3′-terminiRetrovirologyCellular Restriction FactorsAntiretroviral DrugsHybrid CapturePCR WorkstationMagnetic Streptavidin BeadsBiotinylated OligonucleotidesDNA DenaturationDNA Hybridization

Read Article

Cite this Article

Pollpeter, D., Sobala, A., Malim, M. H. Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells. J. Vis. Exp. (143), e58715, doi:10.3791/58715 (2019).

Download .ris file

Copy

Share Video

.

Copy