Rivista
/
Neuroscienze
/
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

DOI:
10.3791/58576-v

•

07:18 min

•

November 09, 2018

•

, , , , ,
1Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis,Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center,Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Capitoli

  • 00:04Titolo
  • 00:37Uptake Assay with Fluorescence-activated Cell Sorting (FACS) Read-out
  • 04:33FACS Analysis
  • 05:41Results: Antibody-mediated Increase of Tau Uptake in BV-2 Cells
  • 06:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.

Tags

Cell-based AssayAntibody-mediated Tau ClearanceMicrogliaAlzheimer’s DiseasePH Dye-Tau AggregatesAnti-Tau AntibodiesBV-2 CellsCell CultureTrypsin EDTACentrifugation

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Laser Capture Microdissection of Drosophila Peripheral Neurons

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Read Article