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Neuroscience

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JoVE Journal Neuroscience
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
 

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Article DOI: 10.3791/58565-v • 08:17 min • February 15th, 2019
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

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Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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Intrathecal Injection Recombinant Adeno-associated Viruses Adult Mice Viral Transgenes Central Nervous System Diseases ALS Treatment Lidocaine Hydrochloride Transient Paralysis Visual Indicator Success Rate Hamilton Syringe Genome Copies Biosafety Hood Sterile Gauze Pelvic Girdle Iodine Based Scrub Ethanol
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