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电子顺磁共振在生物样品环境温度和 77 k 的应用

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

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电子顺磁共振在生物样品环境温度和 77 k 的应用

DOI:

10.3791/58461-v

•

06:45 min

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January 11, 2019

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Hanan B. Elajaili, Laura Hernandez-Lagunas, Kalina Ranguelova, Sergey Dikalov, Eva Nozik-Grayck

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Capitoli

00:04Titolo
01:05RAW 264.7 Cell Phorbol 12-Myristate 13-Acetate (PMA)-Treatment
02:27Superoxide Detection in Buffer or Cell Samples
03:58EPR Measurements on Flash Frozen Lung Tissue
04:57Results: Representative Superoxide Detection
06:21Conclusion

Summary

Traduzione automatica

电子顺磁共振 (epr) 光谱是一种明确的方法来测量自由基。使用选择性自旋探针可以检测不同细胞隔间中的自由基。我们提出了一个实用、有效的方法来收集生物样本, 方便处理、储存和转移用于 epr 测量的样品。

Tags

Electron Paramagnetic Resonance EPR Spectroscopy Redox Biology Reactive Oxygen Species Spin Probes Superoxide Detection 264.7 Cells Krebs-Henseleit Buffer DTPA Superoxide Dismutase CMH PMA Room Temperature 77 K

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