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Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
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JoVE Journal Biochimica
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

DOI:
10.3791/58461-v

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06:45 min

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January 11, 2019

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, , , ,
1Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, 2Bruker BioSpin Corp, 3Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Capitoli

  • 00:04Titolo
  • 01:05RAW 264.7 Cell Phorbol 12-Myristate 13-Acetate (PMA)-Treatment
  • 02:27Superoxide Detection in Buffer or Cell Samples
  • 03:58EPR Measurements on Flash Frozen Lung Tissue
  • 04:57Results: Representative Superoxide Detection
  • 06:21Conclusion

Summary

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Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.

Tags

Electron Paramagnetic ResonanceEPR SpectroscopyRedox BiologyReactive Oxygen SpeciesSpin ProbesSuperoxide Detection264.7 CellsKrebs-Henseleit BufferDTPASuperoxide DismutaseCMHPMARoom Temperature77 K

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