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Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

DOI:
10.3791/58453-v

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05:39 min

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October 30, 2018

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, , ,
1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2MedEngine Oy, 3Department of Pediatrics,Tampere University Hospital, 4Department of Children and Adolescents,Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center,University of Oulu

Capitoli

  • 00:04Titolo
  • 00:35Pulling the Microinjection Needles
  • 01:10Filling the Micropipette Needles
  • 01:49Setting the Microinjector and Electroporator for Immunization
  • 02:40Injection of the DNA Vaccine and Electroporation and Visualization of Antigen Expression
  • 04:04Results: Danio rerio Injected with DNA-based Vaccine
  • 05:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.

Tags

Keywords: Adult ZebrafishDNA-based VaccinesPreclinical ScreeningTuberculosisCost-effectiveInjectionPlasmidMicro ManipulatorElectroporatorAnesthesia

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