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免疫沉淀法研究受体与酶的功能相互作用
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
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免疫沉淀法研究受体与酶的功能相互作用

14,533 Views

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09:40 min

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September 28, 2018

DOI:

10.3791/58433-v

DOI:
10.3791/58433-v

•

09:40 min
September 28, 2018

•

3 Views
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1Pulmonary Department,The Chaim Sheba Medical Center, 2Columbia Center for Translational Immunology, Department of Medicine,Columbia University Medical Center

Tags

Co-immunoprecipitationReceptor-enzyme InteractionCell SignalingProtein DomainsEnzymatic ActivationDMEM293-T CellsTransfectionGFP ExpressionPervanadatePhosphorylationPD-1Lysis BufferProtease InhibitorsSodium OrthovanadateImmunoprecipitation

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Peled, M., Strazza, M., Mor, A. Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes. J. Vis. Exp. (139), e58433, doi:10.3791/58433 (2018).

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