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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

DOI:
10.3791/58407-v

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08:36 min

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November 01, 2018

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, , , , ,
1Department of Microbiology and Infection Control,Osaka Medical College

Capitoli

  • 00:04Titolo
  • 01:06Synthesis of the DENV 3’UTR RNA Standard
  • 03:15Processing of Virus Samples for RT-qPCR
  • 04:19Real-time PCR Analysis
  • 06:01Results: Real-time Quantitative Polymerase Chain Reaction of Virus RNA
  • 07:58Conclusion

Summary

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Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

Tags

Dengue VirusRNA QuantificationReal-time PCRRNA StandardIn Vitro TranscriptionRNA PurificationViral RNA DetectionVirus ResearchDrug ScreeningClinical Diagnosis

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