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Un modelo de novela In Vitro de lesión cerebral traumática por explosión
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December 21, 2018
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1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer,Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering,Imperial College London, 3Department of Bioengineering,Imperial College London, 4Department of Life Sciences,Imperial College London, 5Department of Anaesthetics,Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine,Medical Directorate Joint Force Command
Summary
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Este papel describe un modelo novedoso de lesión de cerebro traumática de la explosión primaria. Un tubo de descarga por aire comprimido se utiliza para exponer en vitro ratón rebanada hippocampal culturas una sola onda de choque. Este es un protocolo simple y rápido de generar una lesión de tejido de cerebro reproducible con un alto rendimiento.
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Campos-Pires, R., Yonis, A., Macdonald, W., Harris, K., Edge, C. J., Mahoney, P. F., Dickinson, R. A Novel In Vitro Model of Blast Traumatic Brain Injury. J. Vis. Exp. (142), e58400, doi:10.3791/58400 (2018).
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