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Quantification of Hypopigmentation Activity In Vitro
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JoVE Journal Biochimica
Quantification of Hypopigmentation Activity In Vitro

Quantification of Hypopigmentation Activity In Vitro

DOI:
10.3791/58185-v

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06:08 min

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March 06, 2019

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, , , ,
1Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology,Korea University, 2JinWoo Bio Co. Ltd

Capitoli

  • 00:04Titolo
  • 00:31Cellular Tyrosinase Activity and Melanin Content Measurement
  • 03:13Fontana-Masson Staining and Analysis
  • 05:02Results: Arbutin-Suppressed Melanogenesis in Melanocytes
  • 05:32Conclusion

Summary

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We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis.

Tags

HypopigmentationIn VitroTyrosinase ActivityB16-F10 MelanocytesMelanin ContentFontana-Masson StainingAnti-melanogenic ActivityCosmetic DevelopmentSkin Agents

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