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人外周血和淋巴组织中原发性 T 细胞突触界面的评价
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06:27 min
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July 30, 2018
DOI:
1Department of Microbiology and Immunology,Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology,Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet,Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center,Thomas Jefferson University
Summary
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该协议描述了一种研究原发性多克隆人 T 细胞通过平面脂质双层形成突触界面的能力的技术。利用该技术显示了从淋巴结和外周血中提取的人原发性 T 细胞的差异突触形成能力。
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Cite this Article
Steblyanko, M., Anikeeva, N., Buggert, M., Betts, M. R., Sykulev, Y. Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue. J. Vis. Exp. (137), e58143, doi:10.3791/58143 (2018).
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