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Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

DOI:
10.3791/58140-v

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06:39 min

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October 03, 2018

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, , ,
1Genetics Branch,Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2LGI Flow Cytometry Core Facility,Center for Cancer Research, National Cancer Institute, National Institutes of Health

Capitoli

  • 00:04Titolo
  • 00:39Recipient Mouse Preparation and Bone Marrow Nucleated Cell (BMNC) Harvest
  • 02:25BMNC Fluorescence-activated Cell Sorting (FACS) and Hematopoietic Stem Cell Transplantation (HSCT)
  • 04:52Results: Representative HSCT Analyses
  • 06:08Conclusion

Summary

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We describe the use of hematopoietic stem cell transplantation (HSCT) to assess the malignant potential of genetically engineered hematopoietic cells. HSCT is useful for evaluating various malignant hematopoietic cells in vivo as well as generating a large cohort of mice with myelodysplastic syndromes (MDS) or leukemia to evaluate novel therapies.

Tags

Keywords: Hematopoietic Stem Cell TransplantationMyelodysplastic SyndromeCancer Stem CellsFunctional AnalysisDisease Initiating CellsAdoptive TransferTotal Body IrradiationBone Marrow Nucleated CellsFlow SortingBiotinylated Anti-lineage AntibodyAPC Conjugated Anti-Sca1 Antibody

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