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Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

DOI:
10.3791/58047-v

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06:43 min

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October 09, 2018

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, , , , ,
1Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research,Children’s Hospital of Philadelphia, 2Division of Plastic Reconstructive Surgery, Center for Fetal Research,Children’s Hospital of Philadelphia

Capitoli

  • 00:04Titolo
  • 00:37Needle Loading
  • 01:30In Utero Injections
  • 05:24Results: Donor Cell Engraftment After In Utero Transplantation (IUT)
  • 06:18Conclusion

Summary

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We describe a protocol for performing an in utero transplantation (IUT) through intravenous and intra-amniotic routes of injection in the murine model. This protocol can be used to introduce cells, viral vectors, and other substances into the unique immune-tolerant fetal environment.

Tags

In Utero TransplantationMurine ModelEmbryologyGene TherapyStem Cell TransplantationIntravenousIntra-amnioticMicro-injectionPregnant MouseSurgical ProcedureLocal AnesthesiaFetal Exposure

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